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ConceptPrice EPIPrice non EPI
Strains 60.00€ 150.00€
DNA 32.00€ 80.00€
Culture Media 55.00€ 55.00€

 

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CM-0016

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Waris-H + Si

medios de agua dulce


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Base price 55,00 €
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Waris-H + Si Medium

(McFadden G.I & Melkonian M., 1986)

#

Component

Stock solution

Addition per 1 Litre stock solution

Addition per 1 Litre culture medium

1

KNO3

100.00 g

1 ml

2

MgSO4 . 7 H2O

20.00 g

1 ml

3

(NH4)2HPO4

20.00 g

1 ml

4

Ca(NO3)2 . 4 H2O

100.00 g

1 ml

5

HEPES

238.31 g

1 ml

6

P-II Metals

 

1 ml

7

Fe-EDTA

 

1ml

8

Vitamins

 

1 ml

9

Soil extract

 

10 ml

10

Na2SiO3 . 9 H2O

28.42 g

5 ml

 

Preparation of PII Metals Solution

 

#

Component

Stock solution

Addition per 1 Litre stock solution

1

EDTA (Titriplex III)*

3.00 g

2

H3BO3

1.14 g

3

MnCl2 . 4 H2O

0.144 g

4

ZnSO4 .  7 H2O

0.021 g

5

CoCl2 .  6 H2O

0.004 g

 

Preparation of Fe-EDTA Solution

 

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

EDTA (Titriplex II)*

5.22 g

2

FeSO4 . 7 H2O

4.98 g

3

1 N KOH

54.00 ml

 

EDTA (Titriplex II) and FeSO4 x 7 H2O is heated for 30 min (100oC); KOH is    added to the cooled mixture

 

Preparation of Vitamins Solution

 

#

Vitamin

Stock solution concentration

Addition per 1 Litre stock solution

1

Vitamin B12

0.20 mg

2

Biotin

1.00 mg

3

Thiamine-HCl

100.00 mg

4

Niacinamide

0.10 mg

 

Preparation of Soil Extract

 

10g of garden-soil is mixed with 120 ml bidistilled water and boiled for 10 minutes. Afterwards it is centrifuged for 10 minutes (low speed), and the supernatant is filtered through a series of membrane filters from 1.2 µm – 0.1 µm pore size. The remaining filtrate is adjusted to 100 ml with bidistilled water. Aliquots of 10 ml are stored frozen. The soil should not be recently fertilized and should not contain too much humus.

 

Preparation of Culture Solution

Add 1 ml of stock solutions 1-10 to 1000 ml of bidistilled water, adjust the pH to 7.0 and autoclave.

* Titriplex II  -   Ethylenediaminetetraacetic acid  -  C10H16N2O8

* Titriplex III  -  Ethylenediaminetetraacetic acid  disodium salt dihydrate  - C10H14N2Na2O8 x 2 H2O

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