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ASP-H

medios salobres


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Base price 55,00 ā‚¬
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ASP-H Medium, modified

(G.I. McFadden & M. Melkonian 1986, Phycologia 25: 551-557)

#

Component

Stock solution

(g . L-1 dH2O)

Addition per 1 Litre culture medium

1

NaCl

 

18 g

2

MgSO4 . 7 H2O

 

5 g

3

KCl

60.00 g

10 ml

4

HEPES

238.31 g

3 ml

5

CaCl2 . 2 H2O

370.00 g

1 ml

6

NaNO3

+ K2HPO4 . 3 H2O

+ Na2CO3

50.00 g

5.00 g

30.00 g

1 ml

7

NTA (Titriplex I)*

NTA dissolves after addition of NaOH pellets and heating

10.00 g

1 ml

8

Fe-EDTA solution

 

1ml

9

Trace Metals

 

1 ml

10

H3BO3

2.97 g

10 ml

11

Vitamins solution

 

1 ml

 

Preparation of Fe-EDTA Solution

 

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

EDTA (Titriplex II)*

5.22 g

2

FeSO4 . 7 H2O

4.98 g

3

1 N KOH

54.00 ml

 

EDTA (Titriplex II) and FeSO4 x 7 H2O is heated for 30 min (100oC); KOH is added to the cooled mixture

 

Preparation of Trace Metal Solution

 

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

ZnCl2

0.33 g

2

MnCl2 . 4 H2O

4.30 g

3

CoCl2. 6 H2O

0.12 g

4

Na/EDTA (Titriplex III)*

25.80g

pH of Trace Metal Solution is adjusted to 7.5 with 10 N NaOH

Preparation of Vitamins Solution

#

Component

Stock solution concentration

Addition per 1 Litre stock solution

1

Vitamin B12

0.20 mg

2

Biotin

1.00 mg

3

Thiamine-HCl

100.00 mg

4

Niacinamide

0.10 mg

pH of this solution should be around pH 7.0

 

Preparation of Culture Solution

 

Add chemicals and stock solutions as indicated above to 1000 ml of bidistilled water. Adjust pH to 7.7 with NaOH and autoclave.

* Titriplex I  -    Nitrilotriacetic acid  -  C6H9NO6

* Titriplex II  -   Ethylenediaminetetraacetic acid  -  C10H16N2O8

 *Titriplex III  -  Ethylenediaminetetraacetic acid  disodium salt dihydrate  - C10H14N2Na2O8 x 2 H2O

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